first step chromatogram

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• High-performance liquid chromatography — An HPLC. From left to right: A pumping device generating a gradient of two different solvents, a steel enforced column and an apparatus for measuring the absorbance. Acronym HPLC Classification Chromatography …   Wikipedia

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• Displacement chromatography — is a chromatography technique in which a sample is placed onto the head of the column[n 1] and is then displaced by a solute that is more strongly sorbed than the components of the original mixture. The result is that the components are resolved… …   Wikipedia